摘要
目的通过检测糖尿病肾脏疾病（DKD）大鼠肾组织Raf激酶抑制蛋白（RKIP）与NF—κB的表达，研究利妥昔单抗（RTX）对DKD大鼠肾组织RKIP表达的影响。方法SD大鼠按随机数字表法分为正常组、DKD模型组、RTX治疗组（2周组、4周组），各20只。常规法检测血糖及24h尿蛋白量。Western印迹法检测各组RKIP的表达。免疫组化观察RKIP和NF—κB的表达。结果DKD模型组血糖及24h尿蛋白升高（均P〈0．01），大鼠肾小球NF—κB表达显著增高（P〈0．01），RKIP表达降低（P〈0．05）。与模型组比较，RTX治疗组大鼠RKIP表达增高（P〈0．05），24h尿蛋白和肾小球NF—κB表达下降（均P〈0．05）。DKD模型组NF—κB与RKIP表达呈负相关。结论RKIP调节NF—κB途径可能在DKD发生发展中有重要作用。RTX在DKD中可能有一定的治疗作用。
Objective To detect the expression of Raf kinase inhibitor protein （RKIP） and NF—κB in renal tissue of diabetic kidney disease （DKD） rats model, and to investigate the effect of rituximab （RTX） on the expression of RKIP in the renal tissue of DKD rats. Methods SD rats were randomly divided into normal group （N）, DKD model group （M） and RTX treatment group （D）. Blood glucose and 24-hour urine protein of rats were determined in three groups. RKIP protein and NF—κB protein were determined by immunohistoehemistry staining. RKIP protein expression was detected by Western blotting. Results Compared with N group, blood glucose, 24-hour urine protein and NF—κB expression in M group increased significantly （all P〈 0.01）, the expression of RKIP in M group decreased significantly （P〈0.05）. Compared with M group, the expression of RKIP increased significantly in D group （P〈0.05）, and 24-hour urine protein and NF—κB expression decreased in D group （all P〈0.05）. NF—κB protein expression was negatively correlated with RKIP expression in M group. Conclusions The NF—κB pathway regulated by RKIP plays an important role in the development and pathogenesis of diabetic nephropathy. Rituximab may have a role in treatment of DKD.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2012年第3期217-221,共5页
Chinese Journal of Nephrology
基金
贵州省省长基金【黔省专合字（2010）80号】
