摘要
从山东地区产的成年鸭中分离出鸭乙肝病毒,人工感染当地1日龄雏麻鸭。用斑点酶法、斑点杂交法及用电子显微镜观察外周血中被感染鸭乙肝病毒不同时期的动态,并对不同感染阶段的肝组织病理变化进行了分析。结果表明,感染3周时鸭体内病毒复制较为活跃,此时肝组织病理损害也较严重。本实验为研究人类乙肝病毒的早期感染尤其是母婴传播过程外周血中乙肝病毒的动态与肝组织病理变化的关系提供了动物模型,为防治乙肝病毒早期感染提供了实验基础。
It is not clear that the relationship between the dynamic change of early HBV infection in peripheral blood and the liver pathology.For this,we inoculated one-day-old ducklings with duck hepatitis B virus (DHBV)isolated from adult ducks of Shandong province.The sera DHBsAg,DHBV DNA and DHBV were determined with Dot immunoenzymy assay(DOTEIA),Dot hybridization and Electron microscope separatly.The results revealed that the replication of DHBV was much more active and the liver pathologic change was much more obvious in the third week postinoculation.The study provided a useful experimental model for early human hepatitis and mother-to-infant transmission of hepatitis B virus.
出处
《山东医科大学学报》
1992年第4期297-299,共3页
Acta Academiae Medicinae Shandong
关键词
乙型肝炎病毒
肝炎
感染
病理学
鸭
Hepatitis B virus
Hepatitis,viral,animal
Infection
Virus replication
Pathology
Ducks
