摘要
目的研究杜仲叶中绿原酸的提取纯化方法。方法采用水提、絮凝脱色、树脂吸附、重结晶工艺提取纯化。结果杜仲叶的水浸提液浓缩后用1%壳聚糖絮凝去杂、活性碳脱色得到进样液,进样液用NKA-9树脂吸附,50%乙醇解吸,洗脱液浓缩后的粗品经甲醇重结晶得到纯度≥97%的绿原酸,提取率≥65%。结论优化完善了杜仲叶中绿原酸的提取纯化方法,为杜仲资源的充分综合利用和产业化开发提供了参考。
Objective To study the optimum extraction and purification process for chlorogenic acid in Eucommia ulmoides Oliv. Leaves. Methods The process was carried out by water extraction, 1% chitosan flocculation, actived char decolorization, NKA- 9 resin adsorption, 50% ethanol desorption and methanol recrystallization. Results The extracting ratio of chlorogenic acid was 68% with purity of 97%. Conclusion An optimum and improved technology for the extraction and purification of chlorogenic acid in Ettcommia ulmoides Oliv. Leaves was investigated. It is helpful to industrial production of chlorogenic acid.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第2期446-448,共3页
Lishizhen Medicine and Materia Medica Research
关键词
绿原酸
提取
纯化
杜仲叶
Chlorogenic acid
Extraction
Purification
Eucommia ulmoides Oliv.
