摘要
目的建立引阳素胶囊中淫羊藿苷的高效液相色谱(HPLC)含量测定方法。方法采用HPLC法。色谱柱Li-chrospher C18(4.6 mm×250 mm,5μm),C18保护柱;流动相为乙腈-20 g/L冰醋酸水溶液(30∶70);检测波长270 nm;流速1.0 mL/min;柱温为室温;进样量10μL。样品加50%(体积分数)乙醇超声提取,浸提液过0.45μm滤膜后测定。结果淫羊藿苷线性范围为10.0-60.0 mg/L,回归方程为C=2.042×10-5A+0.554,r=0.9993,平均回收率为99.8%,RSD为0.65%。结论本法用于引阳素胶囊中淫羊藿苷的含量测定时简便快捷、准确。
Objective To establish an HPLC method for testing the content of icariin in Yinyangsu capsules. Methods An Lichrospher C18 （4.6 mm × 250 mm, 5μm） was used as the column, and column C18 as the protecting column. The mobile phase was a mixture of acetonitrile -20 g/L acetum （30 ： 70） in a flow rate of 1.0 mL/min. The detected wavelength was 270 nm. Column temperature was room temperature and the injection quantity was 10μL. The sample was prepared when we added 50% （V/V） alcohol and then made ultrasonic extraction. Measurement was made after the prepared sample was filtrated on 0.45μm filter paper. Results Icariin in the sample was well separated. The linear range was 10.0- 60.0 mg/L. The linear equation was C = 2.042 × 10^-5A ＋ 0. 554 , while the correlation coefficient was 0. 999 3; the average recovery was 99. 8% and RSD was 0. 65% （n=6）. Conclusion This method can be used in content measurement of Yinyangsu capsules.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2007年第1期109-110,113,共3页
Journal of Xi’an Jiaotong University（Medical Sciences）
