摘要
目的：建立以高效液相色谱法测定益肾灵颗粒中有效成分含量的方法。方法：色谱柱为Phenomenex luna C18（250mm×4．6mm，5μm），流动相为乙腈-0．1％冰醋酸溶液（梯度洗脱），检测波长为310am，流速为1．0mL·min^-1,柱温为20℃。结果：淫羊藿苷、补骨脂素及异补骨脂素3种成分均能达到良好分离，在测定范围内线性良好；平均加样回收率均在99％～101％之间，RSD均小于1，20％。结论：本方法简便、可行、重现性好，可用于益肾灵颗粒的质量控制。
OBJECTIVE： To determine the contents of Icariin, Psoralen and Isopsoralen in Yishenling granule by HPLC. METHODS： The chromatographic separation was performed on Phenomenex luna C18 column （250 mm × 4.6 mm, 5μm） with mobile phase consisted of acetonitrile- 0.1% acetic acid solution （gradient el ution） at a flow rate of 1.0 mL · min^-1.The UV detection wavelength was set at 310 nm, and the column temperature was 20 ℃. RESULTS： Icariin,Psoralen and Isopsoralen in Yishenling granules were well - separated with good linearity within their respective concentration range. And the average recoveries for the three constituents were all within 99%～ 101% with RSD all less than 1.20%. CONCLUSION： The method is simple, feasible and reproducible, and it can be used for the quality control of Yishenling granule.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第6期442-444,共3页
China Pharmacy
