摘要
选取肺癌、肺良性病变患者痰液标本180份(肺癌、肺良性病变各90例),经S液固定、DTT溶液解黏、涂片后,用ABC法以异质性核糖核蛋白(hnRNPA2/B1)抗体作细胞免疫组化染色,对比肺癌组与非癌组(良性肺病变)染色情况。另从我院同期确诊的肺癌患者中随机抽取90例,对比hnRNPA2/B1抗体检测与痰细胞学联合纤支镜活检、刷检诊断肺癌的阳性率。结果:hnRNPA2/B1在肺癌组阳性表达率为47.7%,特异性82.2%,与非癌组(17.8%)相比,差异有统计学意义,P<0.005。与痰细胞病理学联合纤支镜活检、刷检阳性率(45%)相比,差异无统计学意义,P>0.05。初步研究结果提示,hnRNPA2/B1在肺癌的诊断中有一定的参考价值,其阳性率接近于痰细胞学联合纤支镜活检、刷检的阳性率。
One hundred and eighty samples of sputum of inpatients and outpatients who habored malign（90 cases） or benign（90 cases） lung tumor were studied after derided into two groups： lung cancer group and non-lung cancer group. Sputum smears were fixed by saccomanno solution and thinned by DTT, the sputum smears slides were used for immunocytotologic reaction with ABC method, observing the nuclear brown stained and determining the results. Positive rate of hnRNP A2/B1 antibody in epithelial cells from patient＇s sample with lung cancer is 47.7%, compared with its counterpart 17.7 % in non-lung cancer group, P〈0. 005. But there is no sharp difference between the positive rate of cytopathology combined with tracheobronchoscopic biopsy,brushing biopsy and hnRNP A2/B1 antibody ,the positive rate is 45% and 47.7% respectively, P〈0.05. The application of hnRNP A2/B1 in clinical diagnosis of lung cancer is feasible and the positive rate approximate to cytopathology combined with tracheobronchoscopic biopsy, brush biopsy.
出处
《中华肿瘤防治杂志》
CAS
2006年第21期1660-1661,共2页
Chinese Journal of Cancer Prevention and Treatment
关键词
肺肿瘤/诊断
核蛋白类
回顾性研究
lung neoplasms/diagnosis
nucleoproteins
retrospective studies
