摘要
目的 研究两种脂质载体——脂质体和脂质微泡对细胞膜的声孔作用。方法 应用体外培养的乳腺癌细胞SK-BR-3，考察脂质体和脂质微泡在低频超声条件下，对SK—BR-3细胞膜的声孔作用，应用原子力显微镜观察超声后即刻和24h的SK—BR-3细胞表面变化。结果 超声可使细胞丧失贴壁性能，形态变圆，脂质微泡的加入明显增强这种作用。原子力显微镜观察表明，对照组（正常细胞单纯超声照射）细胞膜表面孔道暂时增大，部分细胞膜表面孔道直径大于1μm。2％和5％脂质体＋超声照射组和对照组无明显差别。脂质微泡＋超声照射组对细胞膜结构有明显影响，2％脂质微泡＋超声照射组产生的细胞膜表面孔道直径在1—3μm，24h内可以恢复。5％脂质微泡＋超声照射组产生的细胞膜孔道直径2—4μm，24h内不能完全恢复。结论 2％脂质微泡可以产生较强的声孔作用，使细胞膜上出现可逆性孔道，有利于药物或基因递送到细胞内。
Aim To compare sonoporation effect of two phospholipids-based vectors-liposomes and microbubbles on cultured cell membrane. Methods A breast cancer cell line SK-BR-3 was exposed to ultrasound alone, 2% or 5% liposome ＋ ultrasound and 2% or 5% microbubble ＋ ultrasound, separately. Immediately after the experiment and 24 h after ultrasound exposure, atomic-force microscopy （AFM） scanning was used to observe the membrane change of SK-BR-3 cells. Results After ultrasound exposure, normal SK-BR-3 ceils more or less lost their natural shape, showing elliptic outline with obtuse curved boundary. In groups added with phospholipids-based microbubbles, more obtuse curved boundary of cells was observed. The membrane pores of SK-BR-3 cells had apparent changes after ultrasound exposure. With AFM technique, membrane pores under ultrasound alone or ultrasound with liposomes conditions were enlarged, the diameter of some pores exceeding 1 1μm. But all the membrane pores in these conditions returned to normal appearance after 24 hours. In ultrasound with 2% microbubble condition, most membrane pores were about 1 -3 μm in size and returned to normal appearance after 24 h. In ultrasound with 5% microbubble condition, however, pores of most cell membrane porosity was about 2 -4 μm and did not totally return to normal appearance after 24 h. Conclusion At 2% concentration, phospholipids-based microbubble could enhance ultrasonic sonoporation effect and produce reparable membrane pores on SK-BR-3 cells, which appeared to be a promising vehicle for drug and gene delivery.
出处
《药学学报》
CAS
CSCD
北大核心
2006年第12期1176-1179,共4页
Acta Pharmaceutica Sinica
关键词
脂质体
脂质微泡
原子力显微镜
声孔作用
liposome
phospholipids-based mierobubble
atomic-force microscopy
sonoporation
