摘要
目的探讨熊果酸(UA)对ER(-)、Her-2过表达的人乳腺癌SK-BR-3细胞增殖和凋亡的影响。方法利用噻唑蓝(MTT)比色法观察UA对SK-BR-3细胞增殖的影响。用Hoechst 33258荧光染色及电子显微镜观察凋亡细胞核形态变化。流式细胞仪检测UA对细胞周期的影响;DNA琼脂糖凝胶电泳观察DNA片段的断裂。结果UA明显抑制SK-BR-3细胞的增殖,差异有统计学意义(P<0.05),并呈时间和浓度依赖性。经UA处理后的实验组细胞呈现典型的凋亡核固缩表现,胞核呈致密浓染的颗粒状或块状荧光。流式细胞仪检测发现UA作用48h,后SK-BR-3细胞阻滞在G0/G1期,差异有统计学意义(均P<0.05)。DNA琼脂糖凝胶电泳显示DNA大片段的断裂。结论UA在体外对SK-BR-3细胞具有抗增殖和诱导凋亡的作用。
Objective To investigate the inhibitory and apoptotic the proliferation of human breast cancer SK-BR-3 cells. Methods effect of ursolic acid （UA） on The effect of UA on the proliferation of SK-BR-3 cells was measured by MTT method. Hoechst 33258 fluorescent dying and electron microscope were used to observe UA induced morphological changes. The cell cycle was observed with flow cytometry. DNA fragmentation was observed by DNA agarose gel electrophoresis. Results UA inhibitied the growth of SK-BR-3 cells in a dose-and time-dependent manner. The morphological changes of apoptosis , such as nuclear fragmentation or condensed particles were identified after incubation with UA. Cell cycle analysis revealed that SK-BR-3 cells treated with UA for 48 h were blocked predominantly in G0/G1 phase （P〈0.05）. Large fragments of DNA were observed by DNA agarose gel electrophoresis. Conclusion UA has the effects of inhibition of SK-BR-3 cell proliferation
出处
《苏州大学学报（医学版）》
CAS
北大核心
2009年第1期68-70,73,198,共5页
Suzhou University Journal of Medical Science
关键词
熊果酸
凋亡
细胞增殖
HER-2
ursolic acid
and induction of cell apoptosis. apoptosis
cell proliferation
Her-2
