摘要
目的探讨肿瘤坏死因子-α(TNF-α)与低频超声开放大鼠血脑肿瘤屏障之间的关系。方法应用1MHz低频超声辐照C6胶质瘤大鼠,采用伊文思蓝(EB)法检测血脑肿瘤屏障通透性的变化,应用透射电镜观察脑微血管内皮细胞紧密连接的变化,应用酶联免疫吸附试验(ELISA)法检测脑组织中TNF-α含量的变化。结果低频超声辐照后大鼠血脑肿瘤屏障的通透性逐渐增加,在辐照后1.5h￣3h达高峰,12h恢复至正常水平;透射电镜显示低频超声辐照后,0.5h至9h血管内皮细胞的紧密连接均有不同程度的开放,至12h时关闭;低频超声辐照后大鼠胶质瘤侧脑组织中TNF-α含量逐渐增加,在1.5h￣3h达高峰,12h恢复至正常水平。结论低频超声辐照大鼠脑胶质瘤模型后引起TNF-α量的变化与血脑肿瘤屏障通透性变化的趋势及达到峰值的时间相一致。TNF-α的增加可能是低频超声辐照开放大鼠脑胶质瘤血肿瘤屏障的重要因素之一。
Objective To investigate the relationship of low frequency ultrasound opening blood-brain tumor barrier（BBTB） with the expression of tumor necrosis factor α （TNF- α ） in rat. Methods The permeablility of BBTB was measured by Evans blue（EB） assay, the status of the tight junction（TJ） of brain microvascular endothelial cell was observed with transmission electron microseope（TEM）, and enzyme-linked immunosorbent assays（ELISA） was used to detect the content of TNF- α in the C6 glioma brain tissue treated with 1MHz low frequency ultrasound respectively. Results After low frequency ultrasound treatment, the permeablility of BBTB was increased gradually and reached a peak at 1.5h-3h, then restored to normal level at 12h.TEM revealed that the TJ of brain microvascular endothelial cell was destroyed, opened at 0.5h-9h, then closed at 12h. ELISA revealed that the content of TNF-α in the tumor brain tissue was increased gradually and reached a peak value at low frequency ultrasound treatment for 1.5h-3h, then decreased and restored to normal level at 12h. Conclusion Low frequency ultrasound treatment upregulates the expression of TNF- α in the tumor brain tissue and increases the permeablility of BBTB in C6 glioma rat model, TNF-α might be involved in the process of low frequency ultrasound opening the BBTB.
出处
《解剖科学进展》
CAS
2008年第4期391-394,共4页
Progress of Anatomical Sciences
基金
国家自然科学基金(No.30570650
30670723)
教育部高等学校博士学科点专项科研基金(No.20050159005)
辽宁省自然科学基金(No.20052102)
沈阳市科学技术计划(No.1072033-1-00)
关键词
低频超声
肿瘤坏死因子-Α
血脑肿瘤屏障
大鼠
Low frequency ultrasound
tumor necrosis factor α
blood-brain tumor barrier
rat
