摘要
目的建立稳定的原代大鼠脑微血管内皮细胞培养方法,并在体外探讨不同剂量缓激肽的作用靶细胞,进一步阐释缓激肽开放血脑和血肿瘤屏障的机理。方法运用免疫荧光测定原代培养的脑血管内皮细胞、星形胶质细胞及C6胶质瘤细胞在不同剂量缓激肽作用前后的细胞内钙离子变化,根据给药前后的荧光改变来确定不同剂量缓激肽的作用靶点细胞。结果小剂量缓激肽(终浓度:1μmol/L)可以引起C6胶质瘤细胞内的钙离子水平升高,而只有大剂量(终浓度:10μmol/L^1mmol/L)缓激肽才能触发星形胶质细胞内的钙离子水平升高,脑微血管内皮细胞对大、小剂量缓激肽均无任何反应。结论缓激肽的直接作用靶点是胶质细胞及C6胶质瘤细胞,缓激肽调节脑血管内皮细胞通透性的作用可能需要某些细胞间信使的参与。
Objective To investigate the target cells of bradykinin （BK） and the mechanism of BK that could selectively modulate the blood tumor/brain barrier. Methods The intracellular calcium changes in astrocyte, C6 tumor cell and brain microvascular endothelial cell （BMEC） caused by bradykinin were studied with the fluorescence of Fura - 3 respectively to identify the target cells for different doses of bradykinin. Results Small dose （final conc. 1 μmol/L） of BK could trigger intracellular calcium elevation in C6 tumor cell, but not in astrocyte, whereas only large dose of BK （final conc. 10μmol/L - 1mmol/L） could cause the elevation of calcium in astrocyte, and the intracellular calcium in BMEC did not change no matter small or large dose of BK was added. Conclusion The direct target cells of bradykinin are astrocyte and C6 tumor cell, the modulation of bradykinin on the permeability of BMEC may be involved in some intercellular messengers.
出处
《解剖科学进展》
CAS
2005年第3期191-193,i0001-i0002,共5页
Progress of Anatomical Sciences
基金
国家自然科学基金资助项目(NO.3040014530070756830070268)
关键词
缓激肽
血脑屏障
脑微血管内皮细胞
bradykinin
blood brain barrier
brain microvascular endothelial cell
