摘要
目的探讨用长链PCR技术评价60Co-γ射线辐照灭活伪狂犬病毒(pseudorabies virusPRV),后残余病毒感染性的可行性。方法针对PRV糖蛋白gD基因前后的保守区设计预计产物长短不一的5对引物,用PCR扩增经不同剂量60Co-γ射线照射后的PRV核酸,并同时以细胞感染法做平行对照。结果60Co-γ射线辐射对PRV核酸的破坏有剂量依赖性,随着60Co-γ射线剂量的增加,PRV核酸被破坏程度增加,剂量≥20kGy时完全被灭活。5条不同长度PCR扩增产物中,只有长片段3.9kb的检出与细胞培养结果一致。结论γ射线对PRV核酸的损伤程度随γ射线剂量增加而增大;用长链PCR(3.9kb)来评价经60Co-γ射线辐照灭活病毒后残余病毒的感染性是可行的。
Objective To investigate the feasibility of using Long-PCR to estimate the residue transmissibility after ^60Co-gamma irradiation of the Pseudorabies virus（PRV）. Methods We designed five pairs of primers according to the conservative region of the gD gene to PRV and amplified the extracted nucleic acid after ^60Co irradiation of the PRV. Cell culture was performed at the same time to serve as control. Results The destruction of PRV nucleic acid was dose dependent on ^60Co-gammar radiation. Virus destruction increased with increasing dose of irradiation, with complete viral inactivation at a dose of over 20kGy. Only the detection of 3.9kb fragment was consistent with the result of cell culture. Conclusion γ-^60Co destruction of PRV nucleic acid was depended the dose of irradiation. Using Long PCR fragment （3.9kb） to estimate the residue infectivity was feasible after the irradiation.
出处
《中国输血杂志》
CAS
CSCD
2006年第5期368-371,共4页
Chinese Journal of Blood Transfusion
关键词
Γ射线
病毒灭活
长链PCR感染性
gamma irradiation
virus inactivation
long-PCR
infectivity
