摘要
流行性腮腺炎是儿童常见呼吸道传染病，并发症多为睾丸炎、无菌脑炎等。个别患者甚至因严重的脑炎并发症而死亡［１］。故有必要对患者进行早期病原诊断，以利于早期治疗。腮腺炎病毒ＲＮＡ中小疏水蛋白（ＳＨ）基因作为高变区在不同毒株的分子特性鉴别中比其它基因片段更...
Seventy two clinical specimens of saliva,throat swabs and urine collected from clinical mumps cases were examined by the nested RT PCR, 69 (86.1%) of them were positive. The results showed that the positive rate of the urine specimens (25%) was significant different (P<0.005) from that of the saliva (100%) and the throat swab (96.6%) specimens.The Enders strain of mumps virus was used as the positive control and measles virus,new castle disease virus,influenza viruses (A1,A3,B) and rotavirus were used as the negative controls for confirming the specificity of the method.The sensitivity test of the method indicates that at least 1TCID 50 mumps virus or 1～10pg DNA of cloned SH gene plasmid can be detected.This method can be used for clinical detection of the mumps virus with high sensitivity and specificity.
出处
《中国病毒学》
CSCD
1998年第1期96-100,共5页
Virologica Sinica
关键词
腮腺炎病毒
流行性腮腺炎
聚合酶链反应
RNA
Mumps virus(MuV),Mumps, Nested RT PCR (N PCR),Small hydrophobic(SH)protein gene
