摘要
目的建立六味地黄丸中丹皮酚的电化学检测方法.方法循环伏安（CV）、差分脉冲 （DPV）及紫外和高效液相色谱等.结果在0.1 mol·L^-1的NaOH-NaH2PO4（pH=11）底液中、100 mv·s^-1的扫描速度下丹皮酚在铂电极上有一灵敏的氧化峰,峰电流与其浓度在5.0×10^-6～1×10^-3 mol·L^-1 的范围内呈良好的线性关系,线性回归方程为：I= 0.23-4082.64×C（r=0.998 4）,检出限为1×10^-6 mol·L^-1.应用于六味地黄丸中丹皮酚的检测,结果令人满意.结论该方法准确可靠,操作简便、快速,重现性好,无干扰.
Aim To establish the electric chemistry analysis method to measure paeonol in liuweidihuangwan. Method Cyclic voltammerry, differential pulse voltammetry, UV and HPLC were used. Result 0.1 mol · L^- 1 NaOH-Na H2 PO4 buffer solution （ pH = 11 ）, a sensitive oxidization peak of paeonol is obtained. The peak current is directly proportional to the concentration of paeonol over the range of 1.0 × 10^-5 - 1.0 × 10^-3mol · L -1 with the detection limit of 5 × 10^-6mol · L^-1. Conclusion The method is applied to the determination of paeonol in Liuweidihuangwan with satisfactory results. Conclusion This method is accurate and dependable, easy to operate , have good reappearance and no interference.
出处
《安徽医药》
CAS
2006年第6期430-431,共2页
Anhui Medical and Pharmaceutical Journal
基金
安徽省教育厅青年教书科研资助计划(2005i91167)
关键词
六味地黄丸
丹皮酚
电分析化学
liuweidihuangwan
paeonol
electric chemistry analysis
