摘要
中药材及其制剂,因成分复杂,对其有效成分的测定,目前大多尚未建立适宜的方法。文献报道的测定方法有薄层—分光光度法、薄层扫描法、萃取—分光光度法、柱色谱—分光光度法及高效液相色谱法等,这些方法大多操作繁琐、分析周期较长、且分析的精密度和准确度亦不够理想。近年来,随着流动注射分析(FIA)及化学传感器的发展,中成药中有效成分的分析有了新的发展。本文报道了基于小檗碱流通型传感器的电化学检测器用于含小檗碱中(成)药样品的流动注射分析,方法简便快速,结果准确可靠,样品分析速度可达120次/h。
A description is given for the preparation of flow through sensor and of the incorporation of the sensor into a flow injection analysis system. The parameters affecting the measurement are discussed. An accurate, convenient and rapid method is proposed for the determination of berberine- type alkaloids in various Chinese patent medicines (Coptis chinensis Franch.,Phellodendron chinensis Schneid., Xianglian Wan,Zuojin Wan, Ermiao Wan and Sanmiao Wan). The slope is 54 ～ 60 mV/decade over the concentration range of 10^(-3)～3×10^(-6) mol/L berberine at pH 2～9.5. Direct potentiometry determination of berberine in various samples showed an average recovery of 99.5 ～103.5% and a relative standard deviation of 1.3～ 3.5% at a sampling rate of 120/h.
出处
《药学学报》
CAS
CSCD
北大核心
1991年第4期315-319,共5页
Acta Pharmaceutica Sinica
基金
医学重点科研基金
关键词
小檗碱
中成药
化学分析
Berberine
Coptis chinensis
Phellodendron chinensis
Electrochemical detector
Flow injection analysis
