摘要
在生理pH条件下,应用荧光光谱法研究了丹皮酚与溶菌酶的相互作用.结果表明,丹皮酚与溶菌酶反应生成新的复合物,荧光猝灭机制为静态猝灭,结合力类型主要为疏水作用力,结合距离小于7 nm.最后考察了金属离子Zn2+、Cu2+、Fe3+、Al3+对此反应的影响.
The interaction between paeonol and lysozyme was studied under physiological pH by fluorescence spectroscopy.The results showed that paeonol could form a non-covalent complex with lysozyme.The quenching mechanism between them was suggested as a static quenching process,and the binding force was mainly a hydrophobic force.The distance was less than 7 nm according to the theory of the Fster energy transference.Furthermore,the effect of various metals such as Zn2＋,Cu2＋,Fe3＋,Al3＋ on this interaction was explored.
出处
《郑州大学学报（理学版）》
CAS
北大核心
2012年第4期83-86,共4页
Journal of Zhengzhou University:Natural Science Edition
基金
河南省教育厅基金资助项目
编号12B150031
河南省科技厅基金资助项目
编号102102310321
关键词
丹皮酚
荧光光谱
溶菌酶
相互作用
paeonol
fluorescence spectroscopy
lysozyme
interaction
