摘要
目的探讨手术创伤对大鼠空间学习记忆能力及海马胶原纤维酸性蛋白(GFAP)、S100蛋白表达的影响。方法SD大鼠98只随机分为三组:对照组(A组,n=14)、麻醉组(B组,n=42)、手术组(C组,n=42),B、C组根据麻醉、手术后1d或3d或7d分为3个亚组B1、B3、B7组和C1、C3、C7组,每亚组14只。在相应的时间点用Y型电迷宫测试大鼠的空间学习记忆能力,同时用免疫组织化学方法检测海马GFAP和S100蛋白的表达。结果(1)学习记忆能力测试表明:C1组的刺激电压[(53±8)V]高于正常对照A组[(39±7)V](P<0.01)。C1、C3组大鼠Y型电迷宫测试达到学会标准时总共学习的次数分别为(67±26)次、(60±18)次,比其余各组明显增多(P<0.01)。C7组的学习次数[(41±11)次]与A组[(28±7)次]的差异无显著性(P>0.05)。(2)C1组GFAP、S100的表达增多。结论手术创伤可暂时影响大鼠的空间学习记忆能力,并引起海马GFAP、S100蛋白表达的增加,海马星形胶质细胞可能参与了学习记忆过程。
Objective To study the effects of surgery on space learning capability and the expressions of GFAP and S100 in the hippocampus of rats. Methods Total 98 rats were randomly divided into 3 groups：group A,B and C. In group B anesthesia was induced with fentanyl 0. 2mg/kg and droperidol 5mg/kg but did not perform the surgical injury. In group C all rats underwent splenectomy. Y-maze test was used to observe space learning capability on days 1,3,7 after surgery, respectively. According to the time of Y-maze test, group B and group C were divided into 3 subgroup, B1 ,B3 ,B7 and C1 ,C3 ,C7. Meanwhile the expressions of GFAP and S-100 in the hippocampus were also measured. Results Stimulated voltage used in Y-maze test was increased from （39 ± 7 ） V in controls to （53 ±8）V in C1 （P〈0.01）. The total times when they reached the learning criteria were increased in C1 （67 ±26） and C3 （60 ± 18） compare to in group A （28 ±7） （ P〈0. 01 ）. The immunoreactivities of the GFAP and S-100 were increased in the CA1 area of hippocampus 1 day after operation. Conclusion Acute injury which responsed to surgery can impairs space learning capability of rats, and astrocyte of hippocampus may be involved in learning and memory in rats.
出处
《中国行为医学科学》
CSCD
2006年第4期302-304,共3页
Chinese Journal of Behavioral Medical Science
