摘要
目的观察老年大鼠在Y迷宫训练建立空间记忆后N-甲基-D-天冬氨酸受体（NMDA）2B亚基mRNA在海马区的表达变化。方法用Y迷宫对老年（12月龄）大鼠进行短期强化训练，建立空间记忆后，于即刻，3h，6h，12h，24h，3d每组处死8只老年大鼠，取双侧海马，应用RT-PCR方法检测大鼠海马NM-DA受体2B亚基（NR2B）mRNA的表达变化。结果训练组动物（包括即刻，3h，6h，12b，24h组）海马NR2BmRNA的表达量[分别为（683．83±78．13），（806．00±81．43），（848．83±80．84），（915．17±84．25），（568．53±77．14）]明显高于对照组和假训练组[（398．17±74．76），（418．33±70．17），P〈0．05]，且12h时表达最多。3d组的表达量（430．33±74．41）与对照组和假训练组相比差异无统计学意义。结论NR2B可能参与了学习记忆信号的转导过程，且在记忆训练后12h时表达最多，3d时恢复到基础水平。
Objective To observe the changes of NMDA receptor 2B mRNA expression in hippocampus of the old rats after establishment of spatial memory. Methods The spatial memory was establishment by reinfor- cing training in the old rats（ 12 months）. Then the animals were killed eight rats every group at the immediate ,3, 6,12,24h and 3d after establishment of spatial memory and the two hippocampus were isolated for determination of mRNA of NMDA receptor 2B subunit（NP,2B） expression using RT-PCR technique. Results The training groups （including instant,3h,6h, 12h,24h groups） showed significantly higher expressions of NR2B （683.83±78.13, 806.00±81.43,848.83 ±80.84,915.17±84.25,568.53±77.14） than control group and pseudotraining group （398.17±74.76,418.33±70.17 ） （P 〈 0.05 ）. AT the moment of 12h got the maximum expression. There were not significantly different between 3d group and control and pseudotraining group. Conclusion NR2B maybe participate in the signal transduction during learning and memory processes, and got the most at 12h then recovered after 3d.
出处
《中国行为医学科学》
CSCD
2008年第11期979-981,共3页
Chinese Journal of Behavioral Medical Science
