摘要
目的评价脾切除术大鼠术后空间学习记忆能力的变化。方法 SD大鼠98只,随机分为对照组(A组)、麻醉组(B组)和手术组(C组)。B组腹腔注射芬太尼0．2 mg/kg、氟哌利多5 mg/kg 麻醉,不做手术。C组麻醉下行脾切除术。B、C组随机分为3个亚组(B1、B3、B7组和C1、C3、C7组,n =14),分别于麻醉或术后1、3、7 d进行Y迷宫试验,测试大鼠的空间学习记忆能力,RT-PCR测定海马 TNF-α mRNA、IL-1β mRNA表达,Western-blot测定TNF-α、IL-1β蛋白的表达。结果与A、B1组相比,C1 组大鼠跑动所需的刺激电压增大,Y迷宫的学习次数增多;C3组Y迷宫的学习次数比A、B3组增多 (P<0．01)。C7组学习记忆成绩与A、B7组相似(P>0．05)。与A、B1组相比,C1组海马TNF-α mRNA 及IL-1β蛋白表达增多,C1、C3组海马IL-1β mRNA表达升高(P<0．05)。结论大鼠脾切除术后发生短暂认知功能障碍,可能与海马TNF-α mRNA、IL-1β mRNA表达升高和IL-1β蛋白表达增多有关。
Objective To investigate the effect of acute injury caused by surgery on spatial learning and memory and the underlying mechanism. Methods Ninty-eight healthy SD rats of both sexes weighing 300-350 g were randomly divided into 3 groups： （A） control group received neither anesthesia nor surgery （ n = 14） ; （B） anesthesia group received intraperitoneal fentanyl 0.2 mg·kg^-1 and droperidol 5mg·kg^-1 only （ n = 42） and （C） splenectomy group received splenectomy under fentanyl and droperidol anesthesia （ n = 42） . Y-maze test was performed to assess the ability of spatial learning and memory in group A and on the 1 st, 3 rd and 7 th day after anesthesia or surgery in group B and C. Seven rats were killed immediately after Y-maze test in group A and on the 1 st, 3 rd and 7 th day after anesthesia （in group B） and surgery （in group C）, and hippocampus was immediately removed for determination of the expression of TNF-α and IL-1β mRNA （RT-PCR） and protein （Western blot）. Results The voltage of electric stimulation was significantly higher in group C on the 1 st day after surgery than in group B on the 1st day after anesthesia and in group A. The time spent to learn and remember in Y-maze test was significantly longer in group C on the 3 rd than in group B on the 3 rd day and in group A. The expression of TNF-α mRNA and IL-1β mRNA and protein was significantly higher in group C than in group B on the 1st day and in group A. Conclusion Splenectomy can impair the spatial learning ability for a short time after surgery. The increase in the expression of TNF-α mRNA and IL-1β mRNA and protein in hippocampus may play a role in the mechanism.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2006年第2期144-147,共4页
Chinese Journal of Anesthesiology
关键词
外科手术
学习
记忆
应激
Surgical produres, operative i Learning
Memory
Stress
