摘要
目的；建立双波长高效液相色谱同时测定人血清中维生素A、E（VitA、VitE）浓度的方法。方法：移取血清，加入乙腈乙醇混合溶液及正己烷，旋涡混合、离心，分离正己烷层氮气吹干，残渣用流动相溶解后进样。流动相为甲醇-水（92：8）；流速1mL·min^-1；柱温30℃，紫外检测器在325nm和292nm检测VitA和VitE。结果：VitA保留时间为2．63min。浓度线性范围50～800μg·L^-1，r^2=0．9995，平均回收率为97．3％（RSD〈5%），最低捡出量0．4ng（S／N〉3）；VitE保留时间为7．61min，浓度线性范围2～16mg·L^-1，r^2=0．9999，平均回收率为96．2％（RSD〈6％），最低检出量2豫（S／，N〉3）。40例健康成人血清VitA平均值为（389．0±138．0）μg·L^-1。VitE平均值为（10．2±2．5）mg·L^-1。结论：双波长HPIC检测血清中VitA和VitE浓度的方法灵敏度高，回收率和重现性良好，操作快速、简便。
OBJECTIVE To establish an HPLC assay for the determination of vitamin A（VitA） and vitamin E（VitE） in hu man serum and evaluate the nutritional value of the body＇ s of VitA and VitE. METHODS The serum was extracted with n-hexane after the protein in the serum was deposited with acetonitrile/alcohol. The layer of n-hexane was dried with nitrogen, and the residue was dissolved with mobile phase and determined by HPLC. VitA and VitE were separated on XTerra^TM RP18 5μm,3. 9mm×150 mm column,detected at 292 nm for VitE and 325 nm for VitA. Using methanol / water（92/8） as mobile phase when the column temperature was 30℃. RESULTS The linearity range of calibration curve of VitA was 50 - 800μg·L^-2 （r^2 = 0. 999 5）. The average extracted recovery is 97. 3% ,RSD〈5%. The linearity range of calibration curve of VitE was 2 - 16 mg·L^-1 （r^2 = 0. 999 9）. The average extracted recovery was 96. 2 %, RSD〈6%. The detection limit of VitA was 0. 4 ng and VitE is 2 ng （S/N〉3）. The whole operation could be finished in less than 30 minutes. CONCLUSION The method is sensitive, rapid, accurate and simple.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2006年第4期412-414,共3页
Chinese Journal of Hospital Pharmacy
