摘要
建立快速提取血清中维生素A和运用HPLC测定其含量的方法。取待测血清200μL,加入无水乙醇液200μL,旋涡混合1min,再加入正己烷溶液400μL,旋涡混合3min,离心后取上清液,用高效液相色谱仪测定。色谱条件:分析柱为Kromasil100-5C18(150×4.6mm,5μm);流动相为甲醇∶水(98∶2,V/V);流速为1.2mL/min;检测波长为325nm。血清中维生素A分离良好,浓度在2—80μg/dL范围内呈线性关系,r2=0.9992。平均回收率为98.48%,日内、日间RSD均<10%。所测医学生血清中维生素A的含量男性为(38.65±12.99)μg/dL,女性为(34.17±10.10)μg/dL。该法操作快速、样品处理简便易行,适用于血清中维生素A的快速提取和含量的测定。
200μL serum was added 200μL anhydrous alcohol in a vessel. Shaken and centrifuged for 1 min. After 400μL n-hexane was added,the mixture was shaken and centrifuged for 3 min, vitamin A in serum was rapidly extracted. An analytical column was Kromasil 100-5C18 （150 × 4.6mm, 5μ） ; the mobile phase was methanol： water （98 ： 2, V/V） ; the flow rate was 1.2mL/min; determination wavelength was 325 nm, the vitamin A concentration in serum was determined by HPLC. The mean recovery of vitamin A was 98. 48% and RSD within day and between days were all less than 10%. The linear range of determination of serum vitamin A was 2- 80μg/dl. r^2= 0. 9992. Concentration of serum VA among male and female were （38. 65±12. 99） μg/dL, （34. 17 ± 10. 10）μg/dL respectively. The method is found to be simple and rapid, the will supply for rapid extraction and determination of concentration of vitamin A in serum.
出处
《光谱实验室》
CAS
CSCD
北大核心
2009年第3期635-638,共4页
Chinese Journal of Spectroscopy Laboratory
基金
中国营养学会2005年度营养科研基金(编号:05018)
