摘要
目的:探讨环氧化酶-2(COX-2)与血管内皮生长因子(VEGF)在人翼状胬肉成纤维细胞(HPF)中的关系,以及美洛昔康(Meloxicam)抗HPF增殖的作用和机制。方法:采用免疫组化方法测定翼状胬肉组织中COX-2的表达。体外培养HPF,采用Western法,MTT法,研究在不同浓度的VEGF刺激下,HPF中COX-2蛋白的表达,以及不同浓度的美洛昔康对HPF增殖的影响。结果:翼状胬肉组织中COX-2呈阳性表达。HPF中VEGF浓度的增高可致COX-2蛋白的表达呈增强趋势。当美洛昔康的浓度在75～300μmol/L之间变化时,能明显抑制HPF的增殖,且其抑制强度呈剂量、时间依赖性。结论:HPF中VEGF的浓度升高能增强COX-2蛋白的表达,美洛昔康能够通过拮抗COX-2达到抑制HPF增殖的作用。
AIM： To investigate the association between cycloxygenase-2 （COX-2） expression and VEGF intervention as well as the inhibitory effect of Meloxicarn on the cultured human pterygiurn fibroblasts （HPF）.
METHODS： Expression of COX-2 was measured by imrnunohistochernistry in the cultured HPF from twenty exdsed pterygiurn cases. Expression of COX-2 in HPF was measured by Western blot following the treatment of vascular endothelial growth factor （VEGF） at the different concentrations. In addition, the effect of Meloxicarn on proliferation of HPF was studied by adding the different concentrations into the cultured HPF plates by Mono-nudear cell direct cytotoxidty MTT） reduction assay.
RESULTS： COX-2 expression was present in the cultured HPF. The level of the expression increased following VEGF treatment. The proliferation of the cultured HPF decreased following addition of the different concentrations of Meloxicarn （from 75μmol/L to 300μmnol/L） and the magnitude of the inhibition was dose-time dependent.
CONCLUSION： COX-2 levels in the cultured HPF were positively associated with VEGF stimulation and Meloxicam was inhibitory to HPF proliferation.
出处
《国际眼科杂志》
CAS
2006年第1期5-8,共4页
International Eye Science
