摘要
目的探讨多发伤树突状细胞(DC)变化及临床意义。方法从68例多发伤患者和22例健康人外周血中分别提取DC;通过流式细胞仪分别检测各组的DC数量,DC表面HLA-DR、CD80、CD86表达水平以及DC诱导的T细胞反应性增殖情况。同时检测各组中IL-6I、L-10的浓度。结果多发伤组DC细胞数〔(8.3±2.8)×106/L〕明显低于对照组〔(15.2±4.1)×106/L,P<0.01〕。多发伤组DC表面HLA-DR及CD80、CD86的表达水平与对照组相比明显降低(P<0.01)。DC诱导的T细胞增殖能力对照组明显强于创伤组(P<0.01)。多发伤组血清IL-6、IL-10的浓度显著升高,与对照组比较差异有非常显著性意义(P<0.01)。结论多发伤患者DC数量明显减少且功能下降,这可能与创伤后的免疫功能低下密切相关。
Objective To discuss the change and clinical significance of DC in the multiple traumas. Methods Dendritic cells（DC） were separated from peripheral blood in the healthy volunteers （ 22 cases, control group ） and traumatic patients （ 68 cases, multiple trauma group ）. The numhers of DC and expression levels of HLA- DR, IL - 6 and IL - 10 on DC were detected by CMRF - 44mAb labeling and flow cytometer. The supernatants of every group were collected and the concentrations of IL - 6 and IL - 10 were measured by ELISA. The capacity of DC induced the proliferation of T cells was tested in a mixed leukocyte reaction （ MLR ）. Results The DC counts in multiple trauma group[ （7.8±3.5）×10^6/L] were obviously lower than those in control group[ （15.0±5.4）×10^6/L, P 〈 0.01 ]. The expression levels of HLA-DR, CD80 and CD86 on DC in multiple trauma group were obviously lower than those in control group （P 〈 0.01）. The proliferative capacities of T cell induced by DC in control group were comparatively stronger than those in multiple trauma group（ P 〈 0.01 ）. There was a significant difference in the concentration of IL- 6 and IL - 10 between multiple trauma group and control group （ P 〈 0.01）. Conclusion The number of DC was decreased and function was inadequate in traumatic patients, which might be close correlated with immunosuppression after trauma.
出处
《中国急救医学》
CAS
CSCD
北大核心
2005年第11期804-806,共3页
Chinese Journal of Critical Care Medicine
关键词
多发伤
树突状细胞
免疫
Multiple Trauma
Dendritic cell（DC）
Immunity
