摘要
目的 :通过对淋巴结CD 1α+树突状细胞 (dendriticcells,DC)浸润密度进行定量分析 ,探讨淋巴结CD 1α+树突状细胞在肾癌患者抗肿瘤免疫反应中的作用。方法 :5 9例行肾癌根治性切除术的肾癌患者的淋巴结石蜡标本被重新制备 ,用CD 1α单克隆抗体免疫组化SABC法显示DC的分布并检测其浸润密度。结果 :无转移组中DC浸润密度个数平均为 ( 35 .1± 13.0 ) /mm2 ,有转移组平均为 ( 2 5 .9± 9.6 ) /mm2 ,2组差异有显著性 (P <0 .0 0 1)。结论 :淋巴结中CD 1α+DC的浸润对肾癌患者免疫防御机制的建立有重要作用 ,反映了机体免疫系统的抗肿瘤潜能。其浸润密度反映了该患者的抗肿瘤免疫状态 。
Objective:CD-1α + Dendritic cells(DCs) in lymph nodes form a system of highly efficient antigen-presenting cells. An immunohistochemical study was performed to prove their role in immune responses against cancer. Methods:Paraffin blocks were prepared for staining with antibody against CD-1α in tissue specimens obtained from elective lymphadenectomy in 59 cases of kidney neoplasms. Results:The extent of infiltration by CD-1α + DC was evaluated in two groups according to negative and positive metastasis. Densities of DCs is 35.1±13.0/mm 2 on the average in the negative metastasis;and 25.9±9.6/ mm 2 in the positive metastasis. The test revealed that the differences in population density between the two groups were significant ( P <0.001). Conclusion: DCs may be more directly involved in the host immune reaction against tumor by means of the most important antigen-presenting cells. CD-1α + DCs is important for establishing immunologic defense mechanisms in cases of kidney neoplasms,and the densities of DCs can serve as a useful indicator in assessing prognosis of kidney neoplasms.
出处
《军医进修学院学报》
CAS
2004年第3期233-234,共2页
Academic Journal of Pla Postgraduate Medical School
