摘要
目的 探讨严重多发伤后患者早期外周血树突状细胞 (DC)的变化。方法 自严重多发伤患者 (2 4例 ,多发伤组 )和健康人 (13例 ,对照组 )外周血分离出树突状细胞 ;通过流式细胞仪检测各组的DC数量 (CMRF 4 4标记法 )及DC表面HLA DR、CD80、CD86表达水平以及DC诱导的T细胞反应性增殖。结果 多发伤组DC数量〔 (7 3± 3 4 )× 10 6 /L〕明显低于对照组DC数量〔 (14 1± 5 3)× 10 6 /L〕 ,P <0 0 1。多发伤组DC表面HLA DR及CD80、CD86的表达水平与对照组相比明显下调 (P <0 0 1)。DC诱导的T细胞增殖能力多发伤组明显低于对照组 (P <0 0 1)。结论 严重多发伤患者早期外周血DC数量少 ,功能低下 ,与创伤早期免疫功能低下有关。
Objective To explore the relation of the character of dendtitic cell (DC) variation in patients with severe multiple trauma.Methods DC were purified from peripheral blood of health volunteers(control group,13 cases) and trauma patients (trauma group, 24 cases). The number of DC and the expression levels of HLA-DR and CD80,CD86 on DC were detected by CMRF-44 mAb labelling and flow cytometer. The capacity of DCs to induce the proliferation of T-cell was tested in a mixed leukocyte reaction (MLR).Results The DC counts in trauma group [(7.3±3.4)×10 6/L] were obviously lower than in control groups[(14.1±5.3×10 6/L) (P<0.01 ] .The expression level of HLA-DR and CD80, CD86 of DC on trauma group were obviously down-regulated than in control group(P<0.01).The proliferation of T-cell induced by DC were obviously reduced in trauma group than in control group.Conclusion The number is little and the function is inadequate in peripheral blood DC of patients with severe multiple trauma. The character of alterations in DC may be closely correlated with immunosuppression after severe multiple trauma.
出处
《中华急诊医学杂志》
CAS
CSCD
2004年第4期225-227,共3页
Chinese Journal of Emergency Medicine
