摘要
本文用高效液相色谱法（ＨＰＬＣ）和双波长紫外分光光度法（ＵＶ）平行测定了２ｌ例慢性阻塞性肺病（ＣＯＰＤ）患者的血清茶碱浓度，共１２３例次。两种方法之间有良好的线性关系（ｒ＝０．９７６１，Ｐ＜０．０ｌ），但ＵＶ法较ＨＰＬＣ法测得的血清茶喊浓度低（△Ｃ＝－０．６±１．６ｍｇ／Ｌ，ｎ＝１２３，Ｐ＜０．０５）。药物动力学研究表明：两种方法所得药＋时曲线均符合一房室模型，血药浓度的差异并不影响茶碱的主要药动学参数值及由此预测的给药剂量。Ｋａ为３±５／２．５±２．８ｈ^(－１)；Ｋ为０．４±０．０７／０．１５±０．０５ｈ^(－１)；Ｔ１／２为５．７±２．４／５．０±１．８ｈ；Ｃｌ为０．０９±０．０５／０．０８±０．０４Ｌ／（ｋｇ·ｈ）；Ｄ为１．０±０．６／０．９±０．４ｍｇ／（ｋｇ·ｈ），Ｐ＞０．０５。
23 serum samples of theophylline in 21 chronic abstructive pulmonary diseases (COPD) Patients were anal-ysed by high performance liquid chromatography (HPLC) and ultraviolet spectrophotometry (UV) ,their pharma-cokinetic parameters were compared. Linear regression analysis showed that the two methods were highly correlat-ed with a correlation coefficient of 0. 9761(P<0. 01 ). The mean concentration of theophylline determined by UVmethod was significantly lower than that by HPLC (△C= -0. 6 ±1 . 6, n=123, P<0. 05 ). Serum concentration-time from 21 COPD patients was described as a compertment one model. The significant differences in serum con-centrations didn't change the theophylline major pharmacokinetic parameters and estimated dosages [Ka :(3±5)/( 2. 5 ±2. 8 ) h 1 ; T1/2: ( 5. 7 ±2. 4 ) / ( 5. 0 ±1 . 8 ) h ; K : ( 0. 14±0. 07 )/ (0.15±0. 05 ) h-1; Cl : (0. 09 ±0. 05 ) / (0. 08 ±0.04)L/( kg . h ) D : ( 1.0 ±0. 6 )/( 0.9±0.1 )mg/ (kg . h ) ,P>0. 05].
出处
《中国医院药学杂志》
CSCD
北大核心
1996年第5期195-197,共3页
Chinese Journal of Hospital Pharmacy
关键词
茶碱
高效液相色谱
阻塞性肺疾病
药物浓度
theophylline . high performance liquid chromatography
ultraviolet spectrophotometry
pharma-cokinetics
therapeutic drug monitoring
