摘要
目的 探讨白介素 1 (IL 1 β)和白介素 6 (IL 6 )在大鼠全脑缺血再灌注血管内皮细胞炎症反应过程中的作用。方法 将大鼠随机分为假手术组和脑缺血 3 0min再灌注 1h、3h、6h、1 2h、2 4h、48h、72h组 ,动物模型采用全脑缺血模型三血管阻塞法 ,用免疫组化法检测脑组织缺血再灌注不同时间点IL 1 β和IL 6的表达。结果 IL 1 β和IL 6在假手术组脑组织低水平表达。IL 1 β在脑缺血再灌注 1h表达开始上升 ,6～ 1 2h达峰值 ,2 4h开始下降。IL 6在脑缺血再灌注3h表达开始上升 ,2 4～ 48h达峰值 ,48h后开始下降。结论 IL 1 β和IL 6参于了全脑缺血再灌注脑血管内皮细胞炎症损伤的病理过程。
Objective To study the role of IL-1β and IL-6 in inflammtory reaction of endothelial cell in global cerebral ischemia-reperfusion. Methods The rats were randomly divided into the group as sham operated control group and 1h,3h,6h,12h,24h,48h,72h group.The global cerebral ischemia-reperfusion model was formed by occluding three arteries.The expression of IL-1β and IL-6 after ischemia-reperfusion was evaluated in immunohistochemical method.Results The expression of IL-1β and IL-6 was low in sham operated control group.IL-1β slightly increased at 1h and peaked at 6-12h after reperfusion,then decreased.IL-6 increased in 3h,its peak appeared at 24-48h and then began falling.Conclusion IL-1β and IL-6 are involved in the inflammation of endothelial cell during global cerebral ischemia-reperfusion.It probably gives a new treatment way by interfere with this inflammatory reaction.
出处
《西安医科大学学报》
CSCD
北大核心
2001年第5期432-434,共3页
Journal of Xi'an Medical University(Chinese)
