摘要
利用新生成的ZnS悬浮液与半胱氨酸在碱性条件下,反应生成可溶性半胱氨酸锌络合物,离心分离后用塞曼原子吸收光谱测定溶液中的锌质量浓度即可间接的得到半胱氨酸的含量.研究选择了原子吸收间接测定半胱氨酸的最佳条件和方法,在pH 9.70的2% 硼砂底液中于室温下使半胱氨酸试液与新生成的ZnS悬浮液反应25 min,离心分离后测定上层清液的FAAS响应.在所选定的条件下半胱氨酸测定的线性质量浓度范围为0～80 μg·mL-1,特征质量浓度为0.718 μg·mL-1,检测限为0.76 μg·mL-1.该法用于样品液中的半胱氨酸测定,其相对标准偏差RSD为0.58%,方法的回收率在97%～103%之间.并对测定机理进行了探讨.
Under basic condition, freshly generated zinc sulfide suspension can react with cysteine and a soluble complex of zinc cysteinate formed. Then, indirect determination of cysteine was established by determining the total zinc in solution by Zeeman flame atomic absorption spectrometry. The optimized conditions of indirect determination of cysteine were obtained, at pH 9.70, 2% Na2B4O7 solution, cysteine solution is reacted with ZnS at room temperature for 25 min. Under the selected conditions, the line range of the method for determining cysteine is 0-80μg·mL^-1. The characteristic concentration is 0. 718μg·mL^-1. The detection limit is 1.58μg·mL^-1. The method can be used for determination of cysteine. And the recoveries are 97%-103% and the RSD is 0.58%. At the same time, the mechanism of reaction was discussed.
出处
《浙江工业大学学报》
CAS
2005年第4期362-364,共3页
Journal of Zhejiang University of Technology
基金
浙江省分析测试基金资助项目(04128)
关键词
火焰原子吸收光谱法
半胱氨酸
间接测定
硫化锌
Flame atomic absorption spectrometry
Cysteine
Indirect determination
ZnS
