摘要
目的:研究血小板激活因子(PAF)刺激脑微血管内皮细胞导致血小板在内皮细胞上粘附及WEB,DMPP和粉防己碱的抑制作用. 方法:用[~3H]腺嘌呤标记血小板探讨PAF导致血小板在脑微血管内皮细胞上粘附和药物的抑制作用. 结果:PAF 10—100 nmol L^(-1)显著增加血小板与脑微血管内皮细胞的粘附率,WEB,DMPP和粉防己碱抑制由PAF刺激而导致的血小板在脑微血管内皮细胞上的粘附. 结论:DMPP和粉防己碱能够抑制PAF对脑血管的损害作用.
To study platelet activating factor (PAF) stimulating the platelets to adhere to cultured bovine cerebral microvascular en-dothelial cells (CMEC) and the inhibitory effect of triazelodiazepine (WEB),1,5-bis-(3, 4-dimethoxyphenyl )-tetrahydro-( 4H )-pyran (DMPP), tetrandrine (Tet). METHODS: The platelets adhesion to CMEC and the inhibitory effect of drugs were investigated by [3H]adenine labeling of rabbit blood platelet. RESULTS: The platelet adhesion to CMECwas increased by 36 % vs control after CMEC was stimulated with PAF 10 nmol L-1 for 25 min. WEB 0. 1, 1, 10 mmol L-1 or DMPP 0-1, 1, 10 mmol L-1 or Tet 0.1, 1, 10 mmol L~' inhibited the PAF stimulating platelet adhesion to CMEC by 5. 4 % , 16.3 % , 20.1 %; 13.7%, 19.4%, 22.4%;and 5.5%, 23. 1 % , 32. 6 % , respectively. CONCLUSION: DMPP and Tet inhibited the PAF action in cerebral vascular system.
出处
《中国药理学报》
CSCD
1995年第4期318-321,共4页
Acta Pharmacologica Sinica
关键词
血小板激活因子
血管内皮
粉防己碱
吡喃
药理
platelet activating factor
vascular endothelium
platelet adhesiveness
triazoles
tetrandrine
pyrans
