摘要
在制备Ｐｒｏα＿１（Ⅰ）、Ｐｒｏα＿１（Ⅲ）胶原ｃＤＮＡ探针的基础上，采用斑点及原位分子杂交技术，观察汉防己甲素对矽肺组织中Ⅰ、Ⅲ型胶原基因的ｍＲＮＡ的影响。结果表明染尘２、４个月的矽肺组织中Ⅰ、Ⅲ型胶原ｍＲＮＡ的含量比正常肺组织明显增加（Ｐ＜０．０５），经汉防己甲素治疗后增高的Ⅰ、Ⅲ型ｍＲＮＡ含量显著减少（Ｐ＜Ｏ．０５）。原位杂交显示，正常肺组织杂交颗粒主要分布于肺泡间隔的成纤维细胞中，而矽肺病变组织杂交颗粒分布于细胞性结节及增厚间质的成纤维细胞中。由此认为矽肺组织胶原积聚是由石英粉尘引起胶原基因的表达增强所致，汉防己甲素能直接或间接地抑制胶原基因的转录，从而减少病变组织中胶原蛋白的合成。
ffects of tetrandrine (TT) on types Ⅰ and Ⅲ collagen gene mRNA in lung tissues of silicotic rats were studied with RNA dot blot and in situ hybridizatin by cDNA coding human and mouse Proα_1(Ⅰ)and Proα_1(Ⅲ ) collagen. Results revealed types Ⅰ and Ⅲ collagen gene mRNA content in lung tissues of rats exposed to silica dust for two to four months was obviously greater than that in normal lung tissues(P<0.05),and decreased significantly after treatment with TT (P<0. 05)by dot blot technique. In situ hybridization revealed silver granule,of types Ⅰ and Ⅲ collagen gene mRNA were scattered in fibroblasts of alveolar septa in normal lung tissues, and in cellular nodes and thickened interstitia of silicotic tissues,It suggested aggregation of collagen in silicotic tissues was caused by enhancement of Collagen gene expression,and TT could inhibit directly or indirectly transcription of collagen gene,and thus reduce synthesis of collagen protein in silicotic tissues.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
1995年第1期18-20,共3页
Chinese Journal of Preventive Medicine
基金
国家八五攻关课题
