摘要
目的：研究粉防己碱(Tet)和氯丙嗪(Chl)对人胚肺成纤维细胞胶原与透明质酸(HA)合成的影响,为应用钙拮抗剂防治器官纤维化提供依据.方法:采用[~3H]脯氨酸掺入和放射免疫法分别测定胶原与HA合成.结果:Tet 5—80 μmol L^(-1)和Chl 10—40 μmol L^(-1)均以浓度依赖方式抑制胶原与HA合成.Tet 5—20μmol L^(-1))对细胞无明显毒性却显著抑制胶原与HA合成(P<0.01).结论:Tet在低浓度(5—20μmol L^(-1))时对细胞无明显毒性作用而显著抑制成纤维细胞胶原与HA合成,可望成为治疗器官纤维化的有效药物.
AIM:To study the effects of tetrandrine (Tet) and chlorpromazine (Chl) on synthesis of collagen and hyaluronic acid (HA) in cultured human lung fibroblasts (HLF). METHODS: The synthesis of collagen and HA was assessed by measuring the incorporation of [3H]proline and radioimmunoassay. RESULTS: Both Tet (5-80 μmol L-1) and Chl (10-40 μmol L-1) diminished the collagen synthesis in a concentration-dependent manner. The suppression was aggravated at 36 - 48 h. The HA content in the supernatant of culture also decreased gradually with the increasing dosage of Tet or Chl after 24-h exposure. There was no obvious toxic effect of Tet on HLF cells at 5-20 μmol L-1. CONCLUSION:Tet 5-20μmol L-1 decreased the production of collagen and HA without obvious toxity on HLF,suggesting that Tet could be a hopeful anti-fibrosis drug.
出处
《中国药理学报》
CSCD
1995年第5期412-414,共3页
Acta Pharmacologica Sinica
关键词
粉防己碱
氯丙嗪
胶原
透明质酸
成纤维细胞
tetrandrine
chlorpromazine
collagen
hyaluronic acid
fibroblasts
cultured cells
