摘要
应用双重免疫组化、图象分析及电镜技术对５６例胃癌的神经内分泌（ＮＥ）细胞形态特征及增殖活性进行研究。结果发现胃癌中ＮＥ细胞有明显异型性及侵袭性。细胞形态图象测量显示ＮＥ阳性胃癌细胞和正常胃粘膜ＮＥ细胞在各参数的比较中差异均有高度显著性（Ｐ＜０．０１）；但ＮＥ阳性胃癌细胞与ＮＥ阴性胃癌细胞各参数比较（除浆面积以外）差异均无显著性（Ｐ＞０．０５）。ＣｇＡ／ＰＣＮＡ双重免疫组化结果显示：ＣｇＡ阳性胃癌细胞ＰＣＮＡ同步标记率０～３％；ＣｇＡ阴性胃癌细胞ＰＣＮＡ阳性标记率为３１．９％±１４．７％。上述结果提示：胃癌中ＮＥ细胞在形态学上与其它癌细胞一样，具有恶性肿瘤形态学特征，但这些细胞仅少数处于Ｓ期及Ｇ１期，绝大多数处于Ｇ０期，推测这些处于静止状态不伴ＤＮＡ复制的细胞在某些生物活性肽的刺激下，可重新进入细胞周期进行ＤＮＡ复制，完成细胞的增殖分裂。
Neuroendocrine (NE) cells in 56 cases of GC were observed by using double immunohistochemistry, electron microscopy and image analysis. There was a high significant difference ( P<0. 01) in total of image analysis parameters between NE cells in GC and NE cells in normal gastric mucosa, but there was no significant difference in various parameters between NE positive cells in GC and NE negative cancer cells. It was found in CgA/PCNA double immunohistochemistry that CgA positive GC cells with PCNA incorporation was 0￣3%. By contrast, PCNA reaction was positive in 31.9%±14.7% of CgA negative GC cells. It indicated that NE cells in GC were with morphological features of malignant tumor cells, but there were a few cells in S and G1 phase and most of them in G0 phase.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
1995年第4期261-264,共4页
Chinese Journal of Clinical and Experimental Pathology
关键词
胃癌
神经内分泌细胞
形态特征
增殖
gastric carcinoma
neuroendocrine cell
image analysis
double immunohistochemistry
electron microscopy
