摘要
目的：研究癌基因及抗癌基因在喉癌中的联合表达情况。方法：应用ＬＳＡＢ微波免疫组化方法，检测了ｃｍｙｃ、ｒａｓ 与Ｐ５３ 在３０ 例喉癌及癌旁粘膜，４ 例正常喉粘膜中的共同表达。结果：①ｒａｓ，ｃｍｙｃ 和Ｐ５３ 的阳性表达率在癌组织中分别为８６－７ ％（２６／３０），９６－７％ （２９／３０），６３－３％ （１９／３０）；癌旁粘膜分别为４３－３％ （１３／３０） ，５６－７％ （１７／３０），１０－０％ （３／３０） ；正常喉粘膜中分别为２５－０％ （１／４） ，２５－０％ （１／４） ，０－０％ （０／４） 。癌与癌旁及正常粘膜间差异有显著性（Ｐ＜０－０５） 。②２ 种以上癌基因蛋白产物同时表达阳性者喉癌组织中有２８ 例（９３－３ ％ ）；癌旁组织中１４ 例（４６－７％ ）；而正常喉粘膜中无１ 例共同表达。③喉癌组织中，ｃｍｙｃ ＋ ｒａｓ＋ Ｐ５３，ｒａｓ ＋ ｃｍｙｃ，Ｐ５３ ＋ ｃｍｙｃ 同时表达者分别为５６－７％ ，３０－３％ ，６－７ ％ 。④３ 种基因产物的表达与喉癌的分化程度无关（ Ｐ＞０－０５）。结论：ｃｍｙｃ，ｒａｓ 及Ｐ５３ 基因的改变均参与了喉癌的发生、发展过程。
Aim:To study coexpression of protein products of oncogene ras,c myc and P53 in laryngeal carcinoma,adjacent mucosa and normal mucosa.Methods:By using LSAB microwave immunohistochemical technique,we studied coexpression of protein products of these 3 kinds of oncogenes in 30 cases of laryngeal carcinoma and adjacent mucosa,meanwhile in 4 cases of normal mucosa.Results:The positive rates of ras,c myc and P53 expressions in carcinoma were 86.7%,96 7% and 63.3% respectively,and 43.3%,56 7% and 10.0% in mucosa adjacent to carcinoma respectively,while those in normal controls were 25.0%,25 0% and 0.0% respectively( P <0 05).Expressions of two or three oncogenes were found in 93.3% of carcinoma, 46.7% of adjacent mucosa and 0.0% in normal laryngeal mucosa.Coexpression rates of c myc+ras+P53,c myc+ras,c myc+P53 in laryngeal carcinoma were 56.7%,30.3% and 6.7% ,respectively.There was no correlation between expressions of c myc,ras and P53 and histological grade ( P >0.05).Conclusion:The alterations of c myc,ras and P53 genes are involved in calcinogenesis and development of laryngeal carcinoma.They may play a combined role in laryngeal carcinogenesis.
出处
《河南医科大学学报》
1999年第4期31-33,共3页
Journal of Henan Medical University
关键词
喉肿瘤
癌基因
癌基因蛋白
免疫组织化学
laryngeal carcinoma
oncogene
oncoprotein
immunohistochemistry
