摘要
培养的人表皮细胞异体移植后究竟能否长期存活，因无客观的方法证实，目前争议较大。作者采用三种聚合酶链反应体外ＤＮＡ扩增技术（ＰＣＲ），对培养表皮细胞异体移植于不同创面的存活情况进行了判定。结果在１６例、２７个活检标本中均查到了供者细胞的遗传标记，其中最长一例为移植后９２天。这表明人培养表皮异体移植后可长期存活。作者认为三种ＰＣＲ扩增技术各有其优点及不足，根据具体情况配合使用，可对人培养表皮细胞异体移植后的转归做出明确的判定。
AbstractIt is still controversial whether cultured humanepidermal allografts can survive long or not because ofthe lack of convincing evidence to prove it. In thisstudy, three PCR techniques for the individual identifi-cation were used to test the existence of cultured allo-geneic keratinocytes when it has been transplanted todifferents kinds of wounds. With these methods, thedonor genetic marks were found in 27 biopsies of 16patients who had cultured epidermal cells allografted. The longest biopsy time was 92 days after grafting. The results strongly indicated that the survival time ofcultured epidermal allografts was prolonged. The ad-vantage and disadvantage of using the PCR techniqueswere discussed and the fate of the cultured epidermalallografts could be determined if these techniques areused properly.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1995年第7期387-389,共3页
Chinese Journal of Surgery
基金
国家自然科学基金
