摘要
从猪皮真皮提取胶原,经胃蛋白酶处理后再组建成胶原溶液(20mg/ml),用0.06—0.1％戊二醛交联后,经冻干、漂洗后作家兔皮下埋藏,戊二醛胶原埋藏后的90天,有成纤维细胞侵入和新生的血管。并证明应用0.06％戊二醛交联也可达到预防胶原生物降解。
The collagen was prepared from pig dermis by pepsin solubiligation and reconstituted as a fibrillar suspension of 20 mg/ml with glutaraldehyde concentration to less than 0.1%. After freezing and washing, the preparation was implanted in the rabbit subcutaneus tissue. The tissue exhibited a response characterized by fibroblast infiltration and neovascularization in 90 days.The glutaraldehyde cross-linking concentration as low as 0.06% was found capable of preventing biologic degradation.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
1990年第4期325-327,共3页
Journal of Biomedical Engineering
关键词
胶原
戊二醛
交联
体内支撑物
collagen, glutaraldehyde, cross-linking, support in vivo
