摘要
背景与目的:探讨中药方剂1号对人胃癌细胞SGC-7901体外生长及细胞周期的影响。材料与方法:四甲基偶氮唑蓝(3-[4,5-dimethylthiazol-2-y1]-2,5dipheny1tetrazoliumbromide,MTT)还原法检测中药方剂1号对SGC-7901细胞增殖的影响;流式细胞仪检测细胞周期时相分布。结果:中药方剂1号生药能抑制SGC-7901的增殖。在实验浓度范围内,随着剂量的增加,抑制作用更加明显;同一剂量下,72h内药物作用时间越长,抑制作用越明显。中药方剂1号作用48h后,SGC-7901细胞的G0/G1期和S期的细胞减少,G2+M期的细胞增多。结论:中药方剂1号能够抑制人胃癌细胞SGC-7901的体外增殖,其作用机制可能与干扰细胞周期有关。
BACKGROUND&AIM: To investigate the effects of Prescription of Traditional Chinese Medicine-1(PTCM-1)on the proliferation and the cell cycle change arrest of human gastric carcinoma cell line SGC-7901in vitro. MATERIAL AND METHODS: The proliferation of SGC-7901cells was measured by3-[4,5-dimethylthiazol-2-y1] -2,5dipheny1tetrazolium bromide(MTT)colorimetric assay.The cell cycle phase was analysised by flow cytometry. RESULTS: PTCM-1inhibited the proliferation of SGC-7901cells in dose-dependent manner and in time-dependent manner from0h to72h.After treated by PTCM-1for48h,the SGC-7901cells were accumulated in G 2 +M phase in the cell cycle,and the ratio of cells in G 0 /G 1 ,S phase was decreased. CONCLUSION: PTCM-1can inhibit proliferation of SGC-7901cells.It interferences into the cell cycle may be one of mechinism of its antitumor activity.
出处
《癌变·畸变·突变》
CAS
CSCD
2005年第4期220-223,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
浙江省中医药管理局基金资助课题(No.2000C85)
关键词
中药方剂
胃癌细胞
增殖
细胞周期
prescription of traditional Chinese medicine
human gastric carcinoma cell
proliferation
cell cycle
