摘要
目的 研究人参皂甙 -Rh2 (GS -Rh2 )诱导肝癌Bel -740 4细胞凋亡的作用。方法 应用流式细胞术检测GS -Rh2对肝癌Bel -740 4细胞凋亡的诱导作用以及对细胞周期的影响。结果 流式细胞术证实GS -Rh2 具有诱导凋亡作用 ,细胞凋亡率(AR )随GS -Rh2 浓度增高和作用时间延长而升高 ,当GS -Rh2 浓度由 12 .5 μg/ml增至 5 0 .0 μg/ml时 ,AR由 16.2 %升高至2 7.5 % ,但GS -Rh2 浓度由 5 0 .0 μg/ml进一步增至 10 0 .0 μg/ml后 ,AR未见相应升高 ;GS -Rh 2 可将细胞周期阻滞于G1期。结论 GS -Rh2 能诱导体外培养的Bel -740 4细胞凋亡 ;GS -Rh2 诱导Bel -740
Objective To explore the effect of ginsenoside Rh 2(GS Rh2) in induction of apoptosis on Bel 7404 human hepatoma cell line.Methods The apoptosis rate of Bel 7404 cells and its cell cycle analyzation was determined by flow cytometry(FCM).Results GS Rh 2 induced apoptosis on Bel 7404 cell line in a dose and time dependent manner,When the concentration of GS Rh 2 was increased from 12.5 μg/ml to 50.0 μg/ml,the apoptosis rate(AR) of Bel 7404 cell line elevated from 16.2% to 27.5% accordingly;but when the concentration of GS Rh 2 was increased up to 100 μg/ml,the AR no longer rose;GS Rh 2 was capable of arresting the cell cycle at G 1 phase.Conclusions:GS Rh 2 could induce apoptosis on Bel 7404 cell line in vitro;Apoptosis of Bel 7404 cells induced by GS Rh 2 may be related to arresting cell cycle at G 1 phase.
出处
《实用癌症杂志》
2003年第1期16-18,共3页
The Practical Journal of Cancer
关键词
人参皂甙-Rh2
肝肿瘤
凋亡
GS Rh 2(ginsenoside Rh 2)
Liver neoplasm
Apoptosis
