摘要
用微量热学方法测定了E.coli(T4)生物活性系统在LBG培养基中37 ℃培养的热谱曲线.根据曲线数据,经过拟合得到噬菌体增殖热动力学方程ln[Pt/(1-0.077 4 Pt)]=(2.258+0.053 81) t/min和宿主细胞裂解动力学方程lnPt=(4.889 2-0.066 21) t/min,并根据方程求出了生长速率常数k、抑制因子S和裂解速率常数kL.用量热学方法观察到了T4噬菌体的LIN现象.讨论了该研究结果在研究、生产和噬菌体治疗等方面的意义,以及量热学方法在病毒学领域的应用前景.
The thermogenesis power curves of E.coli(T4)living system at (37 ℃) were determined. From the thermogenesis power curves, growth thermokinetic equations of T4 phage and lysis thermokinetic equations of host cell were fit as ln=(2.258+0.053 81) t/min and ln P_t=((4.889 2)-0.066 21) t/min. The growth constant k, inhibition factor S and lysis constant k_L was obtained. The phenomenon of lysis inhibition (LIN) was observed first time with microcalorimetric method. The significance for these results to apply to research, fermentation production and phage therapy was discussed. The application of microcalorimetric method to viroloy was introduced.
出处
《武汉大学学报（理学版）》
CAS
CSCD
北大核心
2005年第2期209-214,共6页
Journal of Wuhan University:Natural Science Edition
基金
国家自然科学基金资助项目(20373051)
