摘要
The interactions of two kinds of water-soluble porphyrins, Cu (NEAE) andH2PPS, with DNA have been investigated by microcajorimetry and UV spectroscopy.The experimental results show that Cu (NEAE) interact with dsDNA in the outside-boundmode, its binding number is 9～10 base pairs and its binding enthalpy △H＝-24．4kJ．mol-1.There are large enthalpy changes when Cu (NEAE) react with ssDNA. H2PPS can notreact with both dsDNA and ssDAN, which demonstrats that the coulombic force is one ofthe important factors affecting the interactions of porphyrins with DNA.
The interactions of two kinds of water-soluble porphyrins, Cu (NEAE) andH2PPS, with DNA have been investigated by microcajorimetry and UV spectroscopy.The experimental results show that Cu (NEAE) interact with dsDNA in the outside-boundmode, its binding number is 9～10 base pairs and its binding enthalpy △H＝-24．4kJ．mol-1.There are large enthalpy changes when Cu (NEAE) react with ssDNA. H2PPS can notreact with both dsDNA and ssDAN, which demonstrats that the coulombic force is one ofthe important factors affecting the interactions of porphyrins with DNA.
出处
《物理化学学报》
SCIE
CAS
CSCD
北大核心
1996年第6期543-546,共4页
Acta Physico-Chimica Sinica
基金
国家自然科学基金
高等学校博士点专项基金
关键词
卟啉
DNA
微量热法
紫外光谱
相互作用
Porphyrin, DNA, Microcalorimetry, UV spectroscopy, Interaction
