摘要
为深入开发利用T4噬菌体展示系统及T4噬菌体的临床细菌病治疗,应用现代各种分子生物学手段,在蛋白分子和组成结构上对T4噬菌体颗粒的各个重要组成部分进行分析,例如头部、尾部和尾板,描述了其蛋白构成和各种影响因子,并着重介绍了在噬菌体装配中最为重要的DNA的包装。同时针对噬菌体T4的感染与超感染免疫性和感染所导致的细胞裂解和裂解抑制等方面,介绍了这些现象发生的分子机制和影响因素。以期对T4噬菌体的分子装配和侵染机制有更为深刻的了解。
To further develop and utilize T4 phage display and phage therapy,and open the broader prospect for the progress in study of T4 phage,the important components of T4 phage particles was analyzed by molecular biology techniques,such as the head,the tail and the basement.The protein composition of these structure were confirmed.DNA packing which was an important part in the assembly of T4 phage particle was described in detail.Not only the phenomenon of infection and lysis,but also the superinfection exclusion and lysis inhibition in T4 life cycles introduced with molecular mechanism and impact factor.The assembly and infection mechanism of T4 phage would be known much better by these research.
出处
《中国农学通报》
CSCD
北大核心
2011年第30期182-186,共5页
Chinese Agricultural Science Bulletin
基金
山东省自然科学基金"大肠杆菌多价噬菌体尾丝蛋白结构与功能研究"(ZR2009DM009)
关键词
T4噬菌体
组装
感染
细胞裂解
bacteriophage T4
assembly
infection
cell lysis
