摘要
在６、７－Ｖ液体培养基中较为适合黄连细胞生长的蔗糖浓度为．３％，当蔗糖浓度增加到７％时，细胞生长速率开始下降，但小檗碱含量提高到５．２０％．小檗碱产率为４５６．６０ｍｇ／Ｌ（２３天）。基本培养基中随着补加ＮＨ４ＮＯ３浓度的增加，抑制细胞生长愈严重；补加０．８ｇ／ＬＮＨ４ＮＯ３的小檗碱含量比未加的增加约１０％，随着浓度的增加又急剧下降。
Three per cent of Sucrose in 6. 7-V liquied media was proper concentration for cell growth of Coptis Chinensis. When sucrose concentration was raised to 7 st, cell growth rate bagan to decrease,but berberine content was raised to 5. 20 %, berberine yield was 456. 60mg/L (23 days). When additional NH4NO3 was added to the bassic media, the high the concentration of ammonium nitrate, the more serious the inhibitation on cell growth; The berberine content in the concentration of 0. 8g/LNH4NOs was 10 per cent more than that without it. but berberine content was rapidly decreased followed the concentration increasing of ammonium nitrate.
出处
《生物技术》
CAS
CSCD
1994年第3期33-35,共3页
Biotechnology
关键词
黄连
蔗糖
硝酸铵
小檗碱
细胞培养
Coptis chinensis
Sucrose
Ammonium nitrate
Cell suspension culture
Berberine
