摘要
利用野生和家栽中国黄连的幼叶、花葶、花蕾等,接种在含有2,4-D(1mg/L)、KT(1mg/L)、NAA(1mg/L)、琼脂(0.75％)、蔗糖(3％)的6·7-V、ER、MS培养基上,在22±1℃条件下进行暗培养45～55天,诱导出愈伤组织,其中以6·7-V培养基诱导频率较高,ER次之,MS较少,不同外殖体和接种时期的愈伤组织诱导频率亦不相同。幼叶与其诱导形成的愈伤组织次生代谢产物均含有小柏碱、药根碱、巴马亭;花亭除含小柏碱、巴马亭外,药根碱含量甚微,而花葶诱导形成的愈伤组织药根碱含量则显著增加。
The callus were induced by inoculating young leaf, flower stalk and bud of Coptis chinensis (cultigen and wild type) onto 6·7-V, ER and MS medium containing 1 mg/L 2, 4-D, l mg/L KT, Img/L NAA, 0.75% agar and 3% sucrose respectively, cultivated at 22+±l1℃ in dark for 40-55 days. Inducing frequency in three medium were 6·7-V>ER>MS. The frequency of different explantand inoculation period were also variant. The secondary matabolities in both young leaves and their callus contained berberine, jatrorrpizine and palmatine. The flower stalks and their callus also contained the three matabolites. There was a little Jatrorrhizine in flower stalks, but the content increased remarkablly in the callus.
出处
《西南农业学报》
CSCD
1989年第4期51-55,共5页
Southwest China Journal of Agricultural Sciences
关键词
黄连
愈伤组织
次生代谢产物
诱导
Coptis chinensis, Callus, Secondary metabolite, Berberine, Jatrorrhizine, Palmatine
