摘要
硬紫草细胞悬浮培养形成紫草宁及其衍生物时,细胞生长曲线呈扁平的S形。细胞停止生长后,紫草宁及其衍生物大量形成,二者的动态变化呈负相关。测定了此过程中培养液的无机元素和可溶性糖含量、溶氧、pH值以及细胞形态的变化情况,可作为硬紫草细胞大规模培养的参考。
In Lithospermum erythrorhizon cell suspension culture, the cell growth curve was a type of flat 'S'. After the growth of cells almost stopped, shikonin derivatives formed rapidly. The change of shikonin formation had negative relevance to that of cell growth. During the culture, the changes of the contents of soluble sugar and inorganic nurtients, DO and pH values of medium, and the morphology of cells were studied. The results could give an important reference for the large-scale cultures of Lithospermum erythrorhizon cells in future.
出处
《生物工程学报》
CAS
CSCD
北大核心
1994年第1期76-80,共5页
Chinese Journal of Biotechnology
关键词
硬紫草
悬浮培养
紫草宁
衍生物
Lithospermum erythrorhizon, shikonin derivatives, cell suspension cultures, large-scale plant cell cultures.
