摘要
以雷公藤茎段为材料,进行了细胞悬浮培养及植株再生诱导的研究。结果表明:愈伤组织诱导培养实验中MS+2,4-D2mg·L-1+KT1.5mg·L-1+NAA0.02 mg·L-1这个处理组中对愈伤组织的诱导培养较为合适,将获得的愈伤组织置于1/2MS+6-BA1.0 mg·L-1+NAA0.2 mg·L-1液体培养基中,黑暗振荡培养,建立细胞悬浮培养体系,更换6次培养液后,分化出芽;分化芽诱导生根后形成植株。
Taking the stem of Tripterygium wilfordii section as a material, the cell suspension and plant regeneration was studied. The result indicated that the most suitable formula for induction of callus is MS ＋2,4-D2mgoL-1 ＋KT1.5mgoL-1 ＋ NAA0.02 mgoL-1. Obtained callus were subsequently cultured in liquid I/2MS＋6-BA1.0 mgoL-I＋NAA0.2 mgoL-1 for establishing cell suspension system with dark cultures. Alter subculture for 4 times, shoots differentiated from the calli and the rooting plantlets were induced.
出处
《三明学院学报》
2010年第6期577-580,共4页
Journal of Sanming University
基金
福建省教育厅科技项目(JA07170)
关键词
雷公藤
愈伤组织
悬浮培养
分化培养
Tripterygium wilfordii
callus
suspension culture
differentiation culture
