摘要
取生长在26℃、光照强度3000lux、14h光照/日条件下的14日苗龄甜菜子叶,用2.0％Cellulase Onozuka K-10+1.0％ Macerozyme Onozuka R-10+0.5％ Driselase+CPW9M酶液酶解。原生质培养在改良MS+2.4-D1.5mg/L+IAA 0.5mg/L+6BA0.5mg/L培养基中液体浅层培养。培养第7～10天时,先后发生第一和第二次细胞分裂,此后细胞继续增殖形成细胞团,约40天时,形成微愈伤组织。
The colyledons of sugarbeet from two-week-old seedling were soluted by enzyme mixture, which consists of 2.0% Cellulase Onozuka R-10+1.0% Macerozyme Onozuka R-10+0.5% Driselase+CPW 9M. The isolated cotyledon protoplasts were cultured in modified MS media with shallow liquid lager. The first cell division appeared after a week of culture and the second cell division began after ten days of culture and the cell cluster formed in following sequential divisions. The microcalli were obtained after 40 days of culture.
出处
《中国甜菜糖业》
1993年第6期10-12,共3页
China Beet & Sugar
