摘要
分别从草木樨状黄芪胚轴再生苗的上部和下部叶片分离原生质体。来自上部叶片的原生质体培养在P_2培养基(含2,4-D 1.0mg/L)中获得了较高的分裂频率(48.9%)和愈伤组织再生频率(321块/m1),过高和过低的2,4-D对于愈伤组织的再生都是不利的。来自下部叶片的原生质体分裂频率很低,不能形成愈伤组织。小愈伤组织转入固体或液体增殖培养基中均能快速生长。愈伤组织转入分化培养基或继续在液体培养基中振荡培养均能分化出芽,频率达100%。目前已获得了大量的再生植株,部分已移栽成活。
Mesophyll protoplasts were isotaledfrom top and basal leaves of regeneratedshoots derived from hypocotyles of Astra-galus melilotoides.The protoplasts from topleaves cultured in P2 medium(K8P me-dium with 1.0mg/L 2,4-D,0.2mg/LNAA and 0.7mg/L 6-BA)showed highercell division frequency(48.9%)and mi-crocallus formation frequency(321 piecesml of protoplast suspension),and the lat-tet was strongly reduced when the proto-plasts were cultured in media with higheror lower concentrations of 2,4-D.Theprotoplasts from basal leaves rarely under-went division,and no callus was obtain-ed.The protoplast-derived microcalli weretransferred to solid media(G1.G2 andG4)or liquid medium(L1)for furtherproliferation.Under these conditions,themicrocalli continued to grow vigrously.After 2—3 weeks,the calli were transfer-red to differentiation media(D1 D4).On which,10(?) of the calli producedshoots,and the shoots rooted by tranfer torooting medium(R).Now,a large num-ber of reg(?)n(?)reted plantlets were obtainedand some of them survived after transplan-tation into soil.
出处
《实验生物学报》
CSCD
1993年第1期11-17,共7页
Acta Biologiae Experimentalis Sinica
关键词
草芪
叶肉
原生质体
植株再生
Astragalus melilotoides.Mesophyll protoplast.Plant regeneration.
