摘要
试验56份大豆栽培品种。采用幼荚子叶、无菌苗下胚轴和子叶、幼胚悬浮培养细胞作原生质体游离材料。苗期子叶和下胚轴用酶液:1％Hemicellulase HP150,0.4％Cellulase R—10,0.1％Pectolyase Y—23;幼荚子叶用1％Cellulasc R—10,1％Hemicellulase HP150,0.5％Pectinase;悬浮培养细胞用1％Cellulase R—10,0.2％Pectolyase Y—23,2％Driselase游离,4种材料均获得大量原生质体。原生质体培养基均为K8P,分化培养基为MS、B5,培养基中的激素试用了2.4—D,2.4.5—T,NAAIAA、ZT、KT、BA、zip和毒莠定等不同配合和浓度。结果有12份品种的幼荚子叶。下胚轴和苗期子叶的原生质体形成愈伤组织、有不同类型的根分化和绿点出现,分化培养基B5+0.1-0.2mg/l 2.4.5-T+1-1.5mg/l·BA较好。
56 soybean (Glycine max) eultivars were tested. Cotyledon of young pod,hypocotyl and cotyledon of aseptic seedlings,and suspension culture cells from young embryo were taken for materials of protoplast isola- tion. The enzyme solutions isolated hypocotyls and cotyledons of aseptic seedlings were 1% Hemicellulase HP 150,0.4% cellulase Onozuk a R-10,0.1% Pectolyase Y-23; the enzyme solution isolated cotyledons of young pods was 1% Cellulase Onozuka R-10,1% Hemicellulase HP 150,0.5% Pectinase; the enzyme solution isolated suspension culture cells from young embryos was 1% Cellulase Onozyka R-10,0.2% Pectolyase Y-23,2% Driselase. The protoplast medium was K8P. The differentiation medium were MS and B5,in which hormones 2.4-D,2.4.5 -T,NAA,IAA,ZT, KT, BA, zip and picloram were tested. Protoplasts from young pod cotyledons and hypocotyls in 12 soybean cultivars formed calli,and different type roots were differentiated and grcenpoints were appeared on some calli. The differentiation media B5+0.1-0.2mg/l 2.4.5-T+1-1.5mg/l BA were the best.
出处
《大豆科学》
CAS
CSCD
北大核心
1989年第3期301-304,共4页
Soybean Science
