摘要
本研究采用多秆多穗青饲玉米为材料,从基因型筛选、愈伤组织的诱导和继代培养、细胞悬浮培养等方面进行了系统的研究,获得四个胚性愈伤组织无性系。原生质体采用液体浅层培养、看护培养、琼脂糖包埋培养等三种方法。79、55、79-8三个基因型获得了愈伤组织和胚状体,并从79和79两个基因型获得再生植株。有一株移栽到土壤中正常生长、开花、吐丝,经过自交和复杂交获得三个正常结实的果穗,经发芽试验,种子具有发芽能力。
This paper deals with systematic studies on the protoplast culture of multitiller and multi- spike forage maize,including selection of genotypes,induction and subculture of embryogenic calli,cell suspansion culture and protoplast culture.Methods such as liquid culture,nurse cul- ture and agarose bead culture were used for protoplast culture.Four cell clones were obtained. Of which calli and embryoids derived from protoplasts were induced from 79,55 and 79-8 three genotypes,and regenerated plants were acquired from 79 and 79-8.One of the regenera- ted plants,after being transplanted into soil,developed normally with flowering and caring. After self and cross pollinations three ears with normally developed seeds were obtained.These seeds germinared well in germination test.
基金
七.五攻关课题
关键词
青饲玉米
原生质体培养
再生植株
Forage maize
Protoplasr culture
Flowering and seeding of regenerated plant
