摘要
从甜菜下胚轴愈伤组织SC_3和未授粉胚珠愈组织SC_1、SC_2中分离到原生质体,在MSB(2)培养基中进行液体浅层培养。约1周后,开始第一次细胞分裂,2～3周左右形成了小细胞团,SC_3原生质体直接发育成原胚。此时统计植板车约为0.1％～0.2％。培养至第五周,3种基因型原生质体中均出现肉眼可见的愈伤组织,原胚发育成球型胚状体。待愈伤组织长到一定大小时,及时转移到新的固态培养基上增殖后进行各种分化试验。球形胚在原培养基中继续培养,7～8周后形成心形胚状体。
Sugarbeet protoplasts were isolated from hypocotyl - derived callus (SC3) and unpollinated ovule-derived callus(SC1, SC2) respectively. Then the protoplasts were cultured in MSB (2) medium with shallow liquid layer. First cell division was appearance after a week, by 2-3 weeks, callus formed in three genotypes and procmbryoid directly generated from SC3 protoplast. Plating efficiency was about 0.1%-0.2% at this time. Visible callus occured until five weeks altc, proembryo developed into globular embryoids. Transfered callus to solid medium for propagation intimc when they reached a appropriate size, then did differentiation experiments, globular cmbryoids turned into heart-shaped cmbryoids in original medium after 7~8 weeks culture.
出处
《中国甜菜》
1993年第1期1-3,T001,共4页
关键词
甜菜
愈伤组织
原生质体培养
Sugarbcct, Callus, Protoplast culture, Embryoid
