摘要
目的:观察大鼠脑缺血再灌注损伤后肿瘤坏死因子-α(tumornecrosisfactor-alpha,TNF-α)表达及细胞凋亡情况,探讨TNF-α在脑缺血再灌注损伤中的作用。方法:采用大鼠局灶性脑缺血再灌注损伤模型,分为3组:脑缺血再灌注组大鼠大脑中动脉阻塞2h,再灌注2,6,12,24,48,72,96h、假手术组及永久缺血组,分别测定TNF-α表达(用免疫组织化学法)及细胞凋亡数(原位凋亡检测试剂盒)。结果:脑缺血再灌注组TNF-α表达水平犤6h:(15.0±3.21)个/mm2,12h:(47.0±0.87)个/mm2,24h:(49.0±10.3)个/mm2,48h:(44.8±6.9)个/mm2,96h:(37.9±6.4)个/mm2犦、细胞凋亡数犤2h:(33.3±0.8)个/mm2,6h:(56.6±1.6)个/mm2,12h:(72.3±4.2)个/mm2,24h:(86.6±5.5)个/mm2,48h:(96.8±0.9)个/mm2犦明显高于假手术组(P<0.01)及永久缺血组(P<0.05);且TNF-α表达与细胞凋亡数关系密切(r=0.567,P<0.01)。结论:大鼠脑缺血再灌注损伤后TNF-α在神经元迟发性坏死中起着重要作用。
AIM:To observe the expression of tumor necrosis factor alpha(TNF α) and nerve cell apoptosis in rats with ischemia/repefusion(I/R) injury,and investigate the effect of TNF αon cerebral I/R injury. METHODS:Rat models of focal cerebral I/R injury were divided into 3 groups:I/R group[middle cerebral artery occlusion(MCAO) for 2 hours and reperfusion for 2,6,12,24,48,72 and 96 hours],sham operated group and permanent ischamia group.The expression of TNF αwas evaluated by immunohistochemistry,and the number of apoptotic cells was measured by terminal dUTP nick end labeling(TUNEL). RESULTS:The expression of TNF α[6 hours:(15.0±3.21)/mm2,12 hours:(47.0±0.87)/mm2,24 hours:(49.0±10.3)/mm2,48 hours:(44.8±6.9)/mm2,96 hours:(37.9±6.4)/mm2] and the number of apoptotic cells[2 hours:(33.3±0.8)/mm2,6 hours:(56.6±1.6)/mm2,12 hours:(72.3±4.2)/mm2,24 hours:(86.6±5.5)/mm2,48 hours:(96.8 ±0.9)/mm2] in the I/R group were significantly higher than those in the sham operated group(P< 0.01) and the permanent ischemia group(P< 0.05) .There was close relationship between the expression of TNF αand apoptosis(r=0.567,P< 0.01). CONCLUSION:TNF αplays an important role in the late neurological necrosis of rats with I/R injury.
出处
《中国临床康复》
CSCD
2004年第28期6094-6095,共2页
Chinese Journal of Clinical Rehabilitation
基金
江苏省徐州市科委资助课题(x2001071)~~
