The flocculating activity of a novel bioflocculant MMF1 produced by multiple-microorganism consortia MM1 was investigated. MM1 was composed of strain BAFRT4 identified as Staphylococcus sp. and strain CYGS1 identified...The flocculating activity of a novel bioflocculant MMF1 produced by multiple-microorganism consortia MM1 was investigated. MM1 was composed of strain BAFRT4 identified as Staphylococcus sp. and strain CYGS1 identified as Pseudomonas sp. The flocculating activity of MMF1 isolated from the screening medium was 82.9%, which is remarkably higher than that of the bioflocculant produced by either of the strains under the same condition. Brewery wastewater was also used as the carbon source for MM1, and the cost-effective production medium for MM1 mainly comprised 1.0 L brewery water （chemical oxygen demand （COD） 5000 mg/L）, 0.5 g/L urea, 0.5 g/L yeast extract, and 0.2 g/L （NH4）2SO4. The optimal conditions for the production of MMF1 was inoculum size 2%, initial pH 6.0, cultivating temperature 30℃, and shaking speed 160 r/min, under which the flocculating activity of the MMF1 reached 96.8%. Fifteen grams of purified bioflocculant could be recovered from 1.0 L of fermentation broth. MMF1 was identified as a macromolecular substance containing both protein and polysaccharide. It showed good flocculating performance in treating indigotin printing and dyeing wastewater, and the maximal removal efficiencies of COD and chroma were 79.2% and 86.5%, respectively.展开更多
目的建立同时测定双料喉风散中靛蓝和靛玉红含量的高效液相色谱法。方法色谱柱采用Phenomenex Luna C18反相色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.02 mol/L乙酸铵(75∶25,V/V),柱温为30℃,流速为1.0 m L/min,检测波长为289 ...目的建立同时测定双料喉风散中靛蓝和靛玉红含量的高效液相色谱法。方法色谱柱采用Phenomenex Luna C18反相色谱柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.02 mol/L乙酸铵(75∶25,V/V),柱温为30℃,流速为1.0 m L/min,检测波长为289 nm,进样量为10μL。结果靛蓝、靛玉红的质量浓度分别在4.52~45.20μg/m L和0.426~4.260μg/m L范围内与峰面积线性关系良好(r>0.999);平均加样回收率分别为98.89%和95.55%,RSD分别为1.12%和1.28%(n=9)。结论该方法简便、准确、重复性好,可用于双料喉风散的质量控制。展开更多
基金Project supported by the Specialized Research Fund for the Doctoral Program of Higher Education(No.20050247016)the Program forNew Century Excellent Talents in University(NCET-05-0387).
文摘The flocculating activity of a novel bioflocculant MMF1 produced by multiple-microorganism consortia MM1 was investigated. MM1 was composed of strain BAFRT4 identified as Staphylococcus sp. and strain CYGS1 identified as Pseudomonas sp. The flocculating activity of MMF1 isolated from the screening medium was 82.9%, which is remarkably higher than that of the bioflocculant produced by either of the strains under the same condition. Brewery wastewater was also used as the carbon source for MM1, and the cost-effective production medium for MM1 mainly comprised 1.0 L brewery water （chemical oxygen demand （COD） 5000 mg/L）, 0.5 g/L urea, 0.5 g/L yeast extract, and 0.2 g/L （NH4）2SO4. The optimal conditions for the production of MMF1 was inoculum size 2%, initial pH 6.0, cultivating temperature 30℃, and shaking speed 160 r/min, under which the flocculating activity of the MMF1 reached 96.8%. Fifteen grams of purified bioflocculant could be recovered from 1.0 L of fermentation broth. MMF1 was identified as a macromolecular substance containing both protein and polysaccharide. It showed good flocculating performance in treating indigotin printing and dyeing wastewater, and the maximal removal efficiencies of COD and chroma were 79.2% and 86.5%, respectively.
